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Biologicals Products List


The biologicals below are produced in our facility of Mons following written procedure and strict environment control. To have more information on a specific product, please click on the catalog number. To place an order, please check our ordering info.

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Catalog number

Cell line

Number of cells

Fraction

Datasheet
CC-01-10-50 HeLa 5.0 x109 whole cells pellet download

CC-01-10-25

HeLa

2.5 x109

whole cells pellet

 

CC-01-10-10

HeLa

1.0 x109

whole cells pellet

 

CC-01-20-50

HeLa

5.0 x109

nuclear extracts

 

CC-01-20-25

HeLa

2.5 x109

nuclear extracts

download

CC-01-20-10

HeLa

1.0 x109

nuclear extracts

 

CC-01-30-50

HeLa

5.0 x109

nuclei

download

CC-01-30-25

HeLa

2.5 x109

nuclei

 

CC-01-30-10

HeLa

1.0 x109

nuclei

 

CC-01-40-25

HeLa

2.5 x109

cytoplasm

download

CC-01-41-50

HeLa

5.0 x109

cytoplasmic S100 fraction

 

CC-01-42-300

HeLa

Lysate

download

CC-01-43-50

HeLa

5.0 x109

mitochondria

 

HeLa WHOLE CELLS


HeLa cell culture

The HeLa cell line was established from an adenocarcinoma of the cervix in 1952. It is the first continuous human cell line. HeLa cells are grown in sonoperfused fedbatch (cytostat) mode at a constant concentration of 5x106 cells/ml (cell viability: 93%-97%) under GLP conditions in our facility in Mons, Belgium.

HeLa cells

Cells are harvested in exponential phase. Cell pellets are prepared by low speed centrifugation, rinsed with phosphate buffer saline, snap frozen in liquid nitrogen and stored at -120°C. A pellet from 5 x 109cells weights about 20 grams. The cell pellets are not prepared in aseptic conditions and are not intended to be used as seed for a new culture.

Research Use

Our HeLa cell pellets are used by research or production entities worldwide for the study of biochemical processing, high throughput screening or purification of biological material from human origin. For published papers reporting the use of our extract, please check out our references list

Quality Control

Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). NCS used in the culture medium is certified from New Zealand origin.


HeLa Cytoplasmic and Nuclear Extracts


CILBIOTECH, provider of high quality HeLa Nuclear Extract (HNE) expands its activities by making available the cytoplasm prepared from exponentially growing HeLa cells (HeLa Cytoplasmic Extract: HCE) propagated under the sonoperfused cytostat technology (cell concentration remains constant without nutrients limitation).
HCE is a rich source of:
  • complexes (40S, 60S ribosomal subunits and 80S ribosomes etc...)
  • Factors involved in tanslation (eIFs', IRES- and PolyA binding proteins).
  • Factors which play a role on different partsof the mRNA molecule (5'- and 3' UTRs').
It can be used to sudy the regulation of muRNA processing, mRNA polymorphism etc...
Preliminary data suggest that HCE compares favorably with the rabbit reticulocyte lysate for cell-free protein expression.
HCE production automation makes it possible to offer this subcellular fraction of high and reproducible quality at very competitive prices.