Production and exploitation of mammalian cells
Mission : mammalian cells to improve the human health.
CIL BIOTECH s.a. has developed and reduced to practice a range of innovative
and enabling technologies related to the culture of isolated cells in
suspension and of anchorage-dependent cells.
These technologies include :
High cell density culture of cells in suspension with concomittant downstream
processing. This technique, easy to implement, has been successfully applied
for the isolation and the purification of monoclonal antibody (92% recovery – 85% purity) secreted by an hybridoma cell line cultivated during
4 weeks at 22E6 cells/ml.
Serial cultivation of anchorage-dependent cells. Successive enzymatic
detachment of anchorage-dependent mammalian cells from large volume cultures
under sterile conditions as practiced today by the pharmaceutical industry
is expensive and very time consuming. Serial cultivation constitutes an
elegant alternative : anchorage-dependent cells detached using a chelator
and controlled mechanical shear (95% viability) are used to inoculate
freshly added microsupport and the whole growth process is then resumed.
All operations can be automated and take place in the same vessel.
New developments relate to :
The development of the perfused cytostat technology. Growing isolated
cells in agitated suspension to a preset cell concentration and maintaining
them at that concentration, simultaneously perfusing the bioreactor to
provide ample amounts of nutrilites suppresses all inconsistencies related
to batch, fed-batch and perfused modes of cultivation, providing the user
with constant, high quality exponentially growing cells.